Effect of gene constellation and postreassortment amino acid change on the phenotypic features of H5 influenza virus reassortants
Identifieur interne : 001440 ( Main/Exploration ); précédent : 001439; suivant : 001441Effect of gene constellation and postreassortment amino acid change on the phenotypic features of H5 influenza virus reassortants
Auteurs : I. A. Rudneva [Russie] ; T. A. Timofeeva [Russie] ; A. A. Shilov [Russie] ; K. S. Kochergin-Nikitsky [Russie] ; N. L. Varich [Russie] ; N. A. Ilyushina [Russie] ; A. S. Gambaryan [Russie] ; P. S. Krylov [Russie] ; N. V. Kaverin [Russie]Source :
- Archives of Virology [ 0304-8608 ] ; 2007-06-01.
Abstract
Summary.: Reassortants between a low-pathogenic avian influenza virus strain A/Duck/Primorie/2621/2001 (H5N2) and a high-yield human influenza virus strain A/Puerto Rico/8/34 (H1N1) were generated, genotyped and analyzed with respect to their yield in embryonated chicken eggs, pathogenicity for mice, and immunogenicity. A reassortant having HA and NA genes from A/Duck/Primorie/2621/2001 virus and 6 genes from A/Puerto Rico/8/34 virus (6:2 reassortant) replicated efficiently in embryonated chicken eggs, the yields being intermediate between the yields of the avian parent virus and those of the A/Puerto Rico/8/34 parent strain. The reassortant having the HA gene from A/Duck/Primorie/2621/2001 virus and 7 genes from A/Puerto Rico/8/34 virus (7:1 reassortant) produced low yields. A variant of the 7:1 reassortant selected by serial passages in eggs had an amino acid substitution in the hemagglutinin (N244D, H3 numbering). The variant produced yields similar to those of the 6:2 reassortant. A 5:3 reassortant generated by a back-cross of the 6:2 reassortant with the avian parent and having PB1, HA and NA genes of A/Duck/Primorie/2621/2001 virus produced higher yields than the 7:1 or 6:2 reassortants, although still lower than the yields of A/Puerto Rico/8/34 virus. The 7:1, 6:2 and 5:3 reassortants were pathogenic for mice, with the level of virulence close to A/Puerto Rico/8/34 virus, in contrast to the extremely low pathogenicity of the A/Duck/Primorie/2621/2001 parent strain. Immunization of mice with an inactivated 6:2 H5N2 reassortant provided efficient immune protection against a reassortant virus containing the HA and NA genes of a recent H5N1 isolate. The results are discussed in connection with the problem of the improvement of vaccine strains against the threatening H5N1 pandemic.
Url:
DOI: 10.1007/s00705-006-0931-8
Affiliations:
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A reassortant having HA and NA genes from A/Duck/Primorie/2621/2001 virus and 6 genes from A/Puerto Rico/8/34 virus (6:2 reassortant) replicated efficiently in embryonated chicken eggs, the yields being intermediate between the yields of the avian parent virus and those of the A/Puerto Rico/8/34 parent strain. The reassortant having the HA gene from A/Duck/Primorie/2621/2001 virus and 7 genes from A/Puerto Rico/8/34 virus (7:1 reassortant) produced low yields. A variant of the 7:1 reassortant selected by serial passages in eggs had an amino acid substitution in the hemagglutinin (N244D, H3 numbering). The variant produced yields similar to those of the 6:2 reassortant. A 5:3 reassortant generated by a back-cross of the 6:2 reassortant with the avian parent and having PB1, HA and NA genes of A/Duck/Primorie/2621/2001 virus produced higher yields than the 7:1 or 6:2 reassortants, although still lower than the yields of A/Puerto Rico/8/34 virus. The 7:1, 6:2 and 5:3 reassortants were pathogenic for mice, with the level of virulence close to A/Puerto Rico/8/34 virus, in contrast to the extremely low pathogenicity of the A/Duck/Primorie/2621/2001 parent strain. Immunization of mice with an inactivated 6:2 H5N2 reassortant provided efficient immune protection against a reassortant virus containing the HA and NA genes of a recent H5N1 isolate. The results are discussed in connection with the problem of the improvement of vaccine strains against the threatening H5N1 pandemic.</div></front></TEI><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Rudneva, I A" sort="Rudneva, I A" uniqKey="Rudneva I" first="I. A." last="Rudneva">I. A. Rudneva</name></region><name sortKey="Gambaryan, A S" sort="Gambaryan, A S" uniqKey="Gambaryan A" first="A. S." last="Gambaryan">A. S. Gambaryan</name><name sortKey="Ilyushina, N A" sort="Ilyushina, N A" uniqKey="Ilyushina N" first="N. A." last="Ilyushina">N. A. Ilyushina</name><name sortKey="Kaverin, N V" sort="Kaverin, N V" uniqKey="Kaverin N" first="N. V." last="Kaverin">N. V. Kaverin</name><name sortKey="Kochergin Nikitsky, K S" sort="Kochergin Nikitsky, K S" uniqKey="Kochergin Nikitsky K" first="K. S." last="Kochergin-Nikitsky">K. S. Kochergin-Nikitsky</name><name sortKey="Krylov, P S" sort="Krylov, P S" uniqKey="Krylov P" first="P. S." last="Krylov">P. S. Krylov</name><name sortKey="Shilov, A A" sort="Shilov, A A" uniqKey="Shilov A" first="A. A." last="Shilov">A. A. Shilov</name><name sortKey="Timofeeva, T A" sort="Timofeeva, T A" uniqKey="Timofeeva T" first="T. A." last="Timofeeva">T. A. Timofeeva</name><name sortKey="Varich, N L" sort="Varich, N L" uniqKey="Varich N" first="N. L." last="Varich">N. L. 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